New technology can reveal the interaction between proteins and provide a powerful tool for the study of membrane proteins
Creative Biostructure has a wealth of experience and advanced technologies in the field of membrane protein. From the membrane protein production, purification, crystallization and structure determination, one stop service is the solid foundation for drug research. In the field of membrane protein research, they have never stopped.
Recently, researchers have developed a new technique for studying human protein expression
. This technique can be used to track the interaction between membrane proteins and other proteins.
Membrane proteins account for approximately 1/3 of all human proteins, and more than 500 diseases are associated with disability. The difficulty of membrane protein research is to understand its role and its interactions with other proteins.
New technology provides a new tool for examining membrane proteins in human cells in a natural environment. The sensitivity is sufficient to detect the trace changes in the drug, so it has important significance for the research and development of cancer and nerve diseases.
The researchers used a new technique called MaMTH (mammalian membrane two hybrid) to determine the role of CRKII protein in the most common lung cancer—non small cell lung cancer. The CRKII protein interacts with the epidermal growth factor receptor protein, and the gene mutations of the epidermal growth factor receptor can lead to the proliferation of cancer cells.
CRKII is most likely to regulate the stability of mutant epidermal growth factor receptor, and promote tumor growth by promoting signaling transfer or communication between cancer cells. It is found that a combination therapy of these mutant receptors and CRKII may be useful in the treatment of lung cancer.
The researchers took 4 years to improve technique for the protein interactions in yeast, and developed a new MaMTH technology. The next step they will study the mutant proteins in other human diseases.
I believe that Creative Biostructure will also benefit from this technology in near future with the protein engineering